TUTORIALS

The students of the Science Department like to share their knowledge with other scientists. Here you will find a number of lab techniques tutorials that they have produced in various subjects.

Bacterial Culture on Petri Dishes

In this video, we explain how to isolate single bacterial colonies of Escherichia coli on lysogeny broth (LB) agar plates using standard aseptic microbiology techniques.

Techniques demonstrated by Ms Miyu SHU  

Video shot and edited by Dr Guillaume ERARD 

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Making a Standard Solution

In this video, we explain the technique for making up a standard solution of 0.3 mol/L sodium chloride (NaCl) in a final volume of 500mL.

Techniques demonstrated by Ms Anna OKADA 
Video shot and edited by Dr Guillaume ERARD 
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Gram Staining of bacterial isolates

Gram staining is a method of staining used to distinguish and classify bacterial species into two large groups (gram-positive and gram-negative). The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique.

Techniques demonstrated by Ms Ayumu NISHIMURA 
Video shot and edited by Dr Guillaume ERARD
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How to Use a Petrifilm for Bacterial Quantification

Petrifilms are convenient, self-contained culture medium that allow easy sampling, selection, and quantification of bacterial isolates.

Techniques demonstrated by Ms Celine MICHEL 
Video shot and edited by Dr Guillaume ERARD
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Plasmolysis in Onion Epidermal Cells

This video describes how to observe plasmolysis (cytoplasm retracting from the cell wall when the cell looses water) in onion epidermal cells.

Techniques demonstrated by Mr David CALALANG 
Video shot and edited by Dr Guillaume ERARD
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Bromelain Breakdown of Gelatin Bonds

Some enzymes present in some tropical fruits can break down protein. Pineapple has a digestive enzyme called bromelain, while papain comes from the papaya fruit. This experiment investigates the properties of bromelain. Bromelain will break down the gelatin proteins into its amino acid components, hence preventing it from setting.

Techniques demonstrated by Ms Celine ESTEBE 
Video shot and edited by Dr Guillaume ERARD
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Lung Dissection

IB Biology HL students dissected a sheep's lungs and annotated pictures they took, comparing the structures they saw to those presented in their textbooks.

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